產品詳細描述

 14-3-3 Family Antibody Sampler Ki該試劑盒包含充足的一抗和二抗，且每種抗體能夠進行四種免疫印跡（Western blot）反應。

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 β/α Antibody.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using 14-3-3 tau Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human GIST, using 14-3-3 tau Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody.

Western Blotting

Western blot analysis of extracts from various cell types using 14-3-3 ε Antibody.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody #9638.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 β/α Antibody #9636.

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, PC12, and COS cells using 14-3-3 ε Antibody #9635.

Western Blotting

Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb.

Western Blotting

Western blot analysis of extracts from various cell lines using 14-3-3 γ (D15B7) Rabbit mAb.

Western Blotting

Western blot analysis of extracts from various cell lines using 14-3-3 ζ/δ (D7H5) Rabbit mAb.

Western Blotting

Western blot analysis of extracts from various cell lines using 14-3-3 γ (D15B7) Rabbit mAb #5522.

Western Blotting

Western blot analysis of extracts from various cell lines using 14-3-3 ζ/δ (D7H5) Rabbit mAb #7413.

Western Blotting

Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb #5521.

Background

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes which modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).

14-3-3蛋白家族在信號轉導、關卡調控 （ checkpoint control）、凋亡和養分敏感通路中起著關鍵性調節作用(1,2)。14-3-3蛋白是高度保守且廣泛表達的蛋白。至少七種亞型包括β、γ、ε、σ、ζ、τ和η ，它們都在哺乳動物體內被鑒定。最初描述的α和δ 亞型是分別被確認為 β和ζ亞型的***酸化形式(3)。通過它們的氨基末端α 螺旋區域，14-3-3蛋白形成同源或異源二聚體，這些二聚體與很多的蛋白質包括轉錄因子、新陳代謝酶、細胞支架 蛋白、激酶、***酸化酶和其它信號分子相互作用(3,4)。主要通過phospho-Ser/Thr motif促使14-3-3蛋白與它們的靶蛋白相互作用。然而，結合不同的phospho-Ser/Thr motifs和非***酸化依賴的相互作用一樣都已經被觀察(4)。14-3-3蛋白的結合掩飾了其靶蛋白特異性序列，因此，該蛋白調節靶蛋白的定位、***酸化水平、穩定性和分子相互作用(1-4)。14-3-3蛋白也可能誘導靶蛋白構象的改變，從而可調節靶蛋白功能(4,5)。明顯的14-3-3蛋白亞型的時空表達模式在對細胞外信號和藥物的發展和急性期已經被觀察，這就認為盡管14-3-3蛋白亞型的序列相似，但是可能具有不同的功能(4)。數個研究認為14-3-3 蛋白亞型在癌癥和神經綜合征中不同的被調節(2,3)。

12630 SignalFire™ Plus ECL Reagent

12757 SignalFire™ Elite ECL Reagent

6883 SignalFire™ ECL Reagent

7074 Anti-rabbit IgG, HRP-linked Antibody

7720 Prestained Protein Marker, Broad Range (Premixed Format)

7727 Biotinylated Protein Ladder Detection Pack